《植物生理学报》 2010, 46(4): 347-350

LED 不同光质对萝卜愈伤组织诱导、增殖和萝卜硫素含量的影响

刘浩¹, 李胜^1,2,*, 马绍英^1,2, 罗丽媛¹, 薛冲¹, 方艳¹, 张真¹, 刘媛¹

1 甘肃农业大学生命科学技术学院植物细胞工程研究室, 兰州730070; 2 兰州汇通生物科技有限公司, 兰州730000

通信作者：李胜；E-mail: lish@gsau.edu.cn；Tel: 0931-7631547

摘　要：

以白水萝卜无菌苗及其愈伤组织为实验材料, 研究其在 LED 白、红、黄、蓝、绿和蓝红 6 种光质下的愈伤诱导和增 殖。结果表明: LED不同光质下胚轴愈伤组织的诱导效应不同, 诱导率顺序依次为黄光>红光>蓝红光>白光>蓝光>绿光; 蓝 光、黄光和红光有利于子叶愈伤组织的诱导; 子叶诱导愈伤组织的效果较下胚轴好; LED红光下愈伤组织的增殖倍数和萝 卜硫素含量均为最高。

关键词：LED; 光质; 萝卜; 愈伤组织; 诱导; 增殖; 萝卜硫素

收稿：2010-01-01   修定：2010-02-22

资助：教育部新教师基金(20096202120008)。

Effects of Different LED Light Qualities on Callus Induction, Proliferation and Sulforaphane Content of Raphanus sativus L.

LIU Hao¹, LI Sheng^1,2,*, MA Shao-Ying^1,2, LUO Li-Yuan¹, XUE Chong¹, FANG Yan¹, ZHANG Zhen¹, LIU Yuan¹

¹Laboratory of Plant Cell Engineering, College of Life Sciences and Technology, Gansu Agricultural University, Lanzhou 730000,China; ²Lanzhou Huitong Biological Science & Technology Ltd., Lanzhou 730000, China

Corresponding author: LI Sheng; E-mail: lish@gsau.edu.cn; Tel: 0931-7631547

Abstract:

The callus induction and proliferation times of white radish (Raphanus sativus) under 6 kinds of LED light qualities (red, yellow, green, blue, white and blue+red) were investigated in this experiment. Results showed that different LED light qualities had different effects on callus induction of hypocotyls, with the efficiency in order of yellow >red >blue+red >white >blue >green, and the blue light, the yellow light and the blue+red light were all in favor of the callus induction of cotyledons, that more callus was induced from cotyledons than from hypocotyls, and that LED red light was the most effective light quality for callus proliferation and sulforaphane accumulation.

Key words: LED; light qualities; Raphanus sativus; callus; induction; proliferation; sulforaphane